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Effects of PUR on apoptosis. (A) Representative images of TUNEL staining (×200) among groups. (B) Flow cytometric analyses of apoptosis levels among groups. (C) Quantitative analyses of TUNEL staining among groups. (D) Quantitative analyses of apoptosis levels by flow cytometry among groups. (E-G) The protein levels in each group. Bars represent the mean ± SD. * P < .05 vs the NC group, # P < .05 vs the TGF-β1 group, n = 3. NC, negative control; PUR, puerarin; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; TGF-β1, transforming growth factor-β1.

Journal: Sexual Medicine

Article Title: Puerarin alleviates oxidative stress, mitochondrial dysfunction, and apoptosis in corpus cavernosum smooth muscle cells through AKT/Nrf2/HO-1 pathway activation

doi: 10.1093/sexmed/qfag022

Figure Lengend Snippet: Effects of PUR on apoptosis. (A) Representative images of TUNEL staining (×200) among groups. (B) Flow cytometric analyses of apoptosis levels among groups. (C) Quantitative analyses of TUNEL staining among groups. (D) Quantitative analyses of apoptosis levels by flow cytometry among groups. (E-G) The protein levels in each group. Bars represent the mean ± SD. * P < .05 vs the NC group, # P < .05 vs the TGF-β1 group, n = 3. NC, negative control; PUR, puerarin; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; TGF-β1, transforming growth factor-β1.

Article Snippet: For in vitro apoptosis detection, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) labeling was applied to cells with a commercial kit (C1086, Beyotime).

Techniques: TUNEL Assay, Staining, Flow Cytometry, Negative Control